Optimization of Florisynthesis of Silver Nano Particles Using Cordia Sebastena Linn

Synthesis of Silver Nanoparticles [AgNPs] using biological method is widely studied. This mostly involves the extracts of leaves of different plants which resulted in nano/microparticle formation. This was mostly due to the presence of complex phytochemicals. However flower of the plants are found to possess minimal amount of secondary metabolites as well as carotenoid pigments which may serve as a reducing agent. Thus, in this study synthesis of Silver nanoparticles using floral extract of Cordia sebastena and optimization in their synthesis using different pH [5, 7 and 9], concentration of silver nitrate [1mM, 2Mm and 3mM] and floral extract [0.5:10, 1:10 and 1.5:10] was conducted. The characterization of AgNPs was carried using UV-Visible Spectrophotometer, Fourier Transform Infra Red [FTIR] Spectrophotometer and Dynamic Light Scattering [DLS]. Further, the floral extract was screened for the presence of different phytoconstituents. The study showed varied Plasmon resonance ranging from 430 to 470nm. Fourier transform infrared spectrum reveals the presence of phytochemicals which acted as reducing and stabilizing agent. The presence of Alkaloids, flavonoids, Phenols, tannins, cardiac glycosides, terpenoids and pholobatannins were recorded in the extract of the flower of Cordia sebastena. The study confirmed the synthesis of AgNPs with the average particle size of 85.2nm. The optimized condition revealed that the flower extract added with 1: 10 concentration to 2mM silver nitrate at pH9 gives smaller AgNPs.