In-vitro Study of Trachyspermum ammi extract against Diabetes Mellitus

In-vitro Study of Trachyspermum ammi extract against Diabetes Mellitus Godavari Amar Amutha Kuppusamy

Diabetes resulting from the low secretion of insulin and insulin resistance, is a chronic metabolic disorder. Medication from plant materials for treatment of Diabetes Mellitus is advised as good source for novel drug or leading to preparation of novel drug. We investigated the antidiabetic property of fractions and subfractions obtained from the ethyl acetate extract of Trachyspermum ammi seeds. TLC analysis of crude extract was done. Then column chromatography was used to isolate the fractions of T. ammi. Primary screening of fractions was done by Starch-Agar Gel Diffusion assay for identification of Alpha-amylase inhibitors. Then after chromatographic separation of subfractions, their secondary screening was carried out by Glucose-Uptake assay in Yeast cells. Finally the in vitro Anti-diabetic activity of crude and screened subfraction of ethyl acetate extract of T. ammi was evaluated in 3T3-L1 cell lines. The results presented the antidiabetic activity of T. ammi and their fractions along with subfractions. Further in vitro cell line assay also proved the antidiabetic activity of T. ammi. It is suggested that the T. ammi had antidiabetic activity by inhibiting the α-amylase enzyme, also by facilitating the glucose utilization in adipose tissues and skeletal muscles. HIGHLIGHTS TLC analysis of crude extract of Trachyspermum ammi was performed Starch-Agar Gel Diffusion assay for identification of Alpha-amylase inhibitors was done Glucose-Uptake assay in Yeast cells was carried out In-vitro Anti-diabetic activity of ethyl acetate extract of ammi was evaluated in 3T3-L1 cell lines GRAPHICAL ABSTRACT
08 15 2022 5698 https://creativecommons.org/licenses/by-nc-nd/4.0 10.48048/tis.2022.5698 https://tis.wu.ac.th/index.php/tis/article/view/5698 https://tis.wu.ac.th/index.php/tis/article/download/5698/334 https://tis.wu.ac.th/index.php/tis/article/download/5698/334