Isolation and characterization of glucanase producing bacteria from forest tree litter

The main objective of the project is to isolate bacteria from Forest soil and characterization of bacteria producing
Glucanase. Three different bacteria are isolated from the forest soil. The Bacteria are identified using
Biochemical Test. The bacterial strain identified are Bacillus, E. coli and Klebsiella. Two screening for
Glucanase producing bacteria are made. The primary screening involves the modified agar diffusion with Congo
red assay. The clear white zones are observed in the primary screening. The sample 3 has large white zone when
compared to sample 2 and sample 1. The secondary screening involves the Glucanse assay stated by Mendal and
Webber. The bacterial sample 3 produced higher concentration of Glucanase than other two bacteria. The
sample 3 has produced 0.473mg of Glucose after the conversion of cellulose by the Glucanase enzyme.