Evaluation of Compound Hordenine (Hordeum vulgare): In-vitro, Gene Expression, and Molecular Modeling Investigations

Evaluation of Compound Hordenine (Hordeum vulgare): In-vitro, Gene Expression, and Molecular Modeling Investigations Lavanya Athilli Research Scholar, Department of Pharmaceutics, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai - 600117, Tamil Nadu, India. Arumugam Balasubramaniam Professor, Department of Pharmaceutics, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai, Tamil Nadu, India. Nemala Appala Raju Professor, Department of Pharmacognosy, Sultan-ul-Uloom College of Pharmacy, Banjara Hills, Hyderabad, Telangana, India.

This study aims to investigate the effects of a compound named hordenine on cells. We examined its impact on gene expression, inflammation, and cell proliferation. To do this, we took unique steps. We started by examining how effectively cells were developing. To gauge how quickly they were multiplying, we conducted a test. We also counted the number of cells by using a machine. Then, we performed a specialized test to evaluate the inflammatory marker IL-6. We also used a technique called RT-PCR to examine particular genes in the cells. Additionally, we used a computer simulation to examine how hordenine interacts with the specific protein MMP1. The findings demonstrated that even a modest amount of hordenine significantly aided cell growth. It also diminished. The stuff called hordenine helped lower inflammation more than another thing called rotenone. Cells treated with hordenine made less of something called IL-6. When we looked at certain genes in skin cells that were treated with hordenine, they seemed more active. Hordenine also binds well to a protein with an energy of -4.5 Kcal/mol. Because of all this, we think hordenine is good for cells. It helps cells grow and can triplicate when they're not working well.
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