SPENT GREEN TEA EXTRACT BASED FUNGAL GROWTH MEDIA DEVELOPMENT

We devised a media from Spent Green Tea Extract (SGTE) and tested its proficiency using two yeast strains; Saccharomyces cerevisiae ATCC 9763 and Candida albicans ATCC 10231, and two filamentous fungal strains Aspergillus brasilensis ATCC 16404 and Penicillium chrysogenum MTCC 5108. No morphological and microscopic differences were observed in SGTE agar medium, when compared with Pottato Dextrose Agar (PDA) media. The absorbance for cell densities in SGTE broth was found to similar in comparison to PDA. Crude SGTE was evaluated for its Minimal Inhibitory Concentration (MIC) and a concentration of 1 mg/mL was found to be effective for Bacillus sp. PVMX4 and a concentration of 7 mg/mL was effective for E. coli ATCC 8739. Bactericidal assay of SGTEA revealed that the medium selectively supported the growth of fungi, wherein PDA failed to inhibit the growth of heterotrophic bacteria from soil samples.