SCREENING OF ANTI-PEPTIC ULCER ACTIVITY OF JASMINUM SAMBAC

Objective: The aim of this study was to investigate the antiulcer activity of callus derived metabolites.
Material and Method: The effect of different (2,4-Dicloro-Phenoxyacetic Acid(2,4-D), Indole- 3-Acetic
acid, (IAA), Indole-3-Butyric Acid(IBA), Naphthalene Acetic Acid(NAA)) growth hormones and their
combination on callus formation, callus texture and weight (fresh and dry) of Jasminum sambac were
investigated. In this investigation revealed that the best callus performance seen in individual MS medium
containing 1.5 mg/L 2,4-D and combination 1.5 mg/L 2,4-D along with 1.5 mg/L IAA. Antiulcer activity was
studied using the pylorus ligation method. A dosage of 200mg/kg b.w used for albino wistar rats was selected
from the LD50 study.Ulcer inhibition and ulcer score, gastric volume, pH, free acidity, total acidity and pepsin,
carbohydrate and protein ratio were analyzed.
Result and Discussion: Plant has show higher activity in percentage of callus formation in MS media
supplemented with single hormone of 1.5 mg/L 2,4-D. The friable callus was observed in single and combination
of the hormone concentration. The callus extract also comprehensively decreased the gastric volume, free and
total acidity, and improved the pH of the gastric fluid, proving its antisecretory activity. The results of this
study showed that the surveyed extracts of the investigated callus exhibited potent antiulcerogenic activity and
dose related activity.