Direct regeneration and in vitro flowering of Scoparia dulcis L

This study reports a simple micropropagation protocol and thereby rapid multiplication of the useful medicinal plant- Scoparia dulcis L. Single node explants were inoculated on basal MS medium containing 3% (w/v) sucrose, supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), kinetin (KN), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and Naphthalene acetic acid (NAA) for direct plant regeneration. Maximum numbers of shoot (~22) were observed on the medium containing 0.5 mg/l BAP and 0.25 mg/l IAA after four weeks of culture. Regenerated shoots were separated and rooted on half strength MS medium supplemented with 0.5 mg/l of IBA alone for three weeks. Simultaneous regeneration of shoots and
roots and in vitro flowering were achieved from the nodal
explants on MS medium supplemented with 0.5 mg/l KN
and 2.0 mg/l IAA. Well-developed complete plantlets
were transferred on to specially made plastic cup
containing soil rite. Acclimatized plantlets were
successfully grown in garden soil.