Development of Analytical Methods for the Determination of Flutamide in Bulk Drug and its Pharmaceutical Formulation

Abstract: A method was developed for estimation of flutamide in bulk drug and its tablet
dosage form by using methanol as a solvent and shows absorbance maxima at 330 nm and
410 nm respectively.This present work deals with the development of two spectrophotometric
methods. The first method is based on diazotization and coupling method using
phloroglucinol (METHOD I). The second one is based on formation of schiff’s base method
using vanillin (METHOD II). In the both methods we are used Perkin Elmer EZ301- UV
visible double beam spectrophotometer. Validation study reveals that the methods are specific. Flutamide obey Beer's law in the concentration ranges used for the methods. Validation studies are statistically significant as all the statistical parameters are within the acceptance range (% COV< 1.0 and S.D. <1.0) for both accuracy and precision study. The methods are simple, rapid, accurate, precise, reproducible, and economic and can be used for routine quantitative analysis of Flutamide in pure and tablet dosage form