ANTIMICROBIAL ACTIVITY OF A PROTEASE INHIBITOR ISOLATED FROM THE RHIZOME OF CURCUMA AMADA

ANTIMICROBIAL ACTIVITY OF A PROTEASE INHIBITOR ISOLATED FROM THE RHIZOME OF CURCUMA AMADA Sangeetha R Padmini R
Objective: Protease inhibitors (PIs) are effective antimicrobial agents, and this study was aimed to study the antibacterial efficacy of a PI isolated from the rhizome of Curcuma amada.Methods: A proteinaceous protease inhibitor was isolated from the rhizome of C. amada and purified by Sephadex G-50 gel permeation chromatography. The purified inhibitor was denoted as Curcuma amada protease inhibitor (CAPI). The antibacterial effect of CAPI against Gram-positive and Gram-negative bacteria, the minimal inhibitory concentration (MIC), and minimal bactericidal concentration (MBC) of CAPI was studied in vitro, and the membrane disruption activity of CAPI was also analyzed.Results: CAPI was effective against both Gram-positive and Gram-negative bacteria, with slightly higher concentrations required for Gram-negative bacteria. The MIC ranged from 75 to 100 μg/ml and the MBC ranged from 100 to 125 μg/ml of CAPI. The study of membrane disruption by CAPI revealed the release of cell contents, namely, reducing sugars and proteins from the bacterial cell.Conclusion: A PI was effectively isolated from the rhizome of C. amada, and the isolated inhibitor proved to be a promising antibacterial agent.
09 01 2017 131 133 10.22159/ajpcr.2017.v10i9.18257 https://innovareacademics.in/journals/index.php/ajpcr/article/view/18257 https://innovareacademics.in/journals/index.php/ajpcr/article/viewFile/18257/12250 https://innovareacademics.in/journals/index.php/ajpcr/article/viewFile/18257/12250