Factors Influencing Rifampicin Autoinduction in Adult Pulmonary Tuberculosis Patients

Chitrakadi Churna is a most trusted oral ayurvedic formulation among the ayurvedic medicines; plumbago
zeylanica is one of the constituent of Chitrakadi Churna. The present study summarizes fingerprinting approach
based on high performance liquid chromatography for Chitrakadi Churna formulation. The Two laboratories
made batches and two marketed batches were taken in this study to estimate the % of plumbagin in this
indigenous formulation. The selection of a suitable chromatographic system, the screening for important
parameters to method validation, and an integrated and universal HPLC fingerprint approach was performed; this
improved the separation quality of the fingerprint. The detection wavelength of plumbagin was found to be 331
nm. The results of the method validation, based on the relative standard deviation of relative retention times and
relative peak areas were acceptable. Calibration curves showed good linear regression (R2 > 0.9989) within test
range. The LODs and the LOQs for the plumbagin were was 0.317mg/ml and 1.0632mg/ml mg/ml .This reported method proves its usefulness for chemical standardization of crude drug and Chitrakadi Churna formulation by means of estimation of plumbagin