Induction of Diploid Androgenesis in the Stinging Catfish, Heteropneustes fossilis

Induction of Diploid Androgenesis in the Stinging Catfish, Heteropneustes fossilis J. G. Christopher School of BioSciences and Technology VIT University Vellore 632 014 India A. G. Murugesan SPK Centre for Environmental Sciences Manonmaniam Sundaranar University Alwarkurichi 627 614 India N. Sukumaran School of Life Sciences Vels University Chennai 600 117 India Abstract

This study reports the results on induced diploid androgenesis in the Indian catfish, Heteropneustes fossilis . Ultraviolet (UV) irradiation was used to inactivate maternal genome of H. fossilis . Complete inactivation of maternal genome was recorded at 12,500 ergs/mm 2 . These genome‐inactivated eggs of H. fossilis were inseminated with conspecific sperm. The sperm suspension was diluted to 1 × 10 7 sperm ml/L in Hank's Balanced Salt Solution. Egg viability was assessed for different exposure durations at fertilization, hatching, haploidy, and diploidization. Majority of the larvae derived from irradiated eggs had abnormal appearance. Complete inactivation of maternal genome was detected by haploid syndrome and confirmed by chromosome counting ( n = 29). These eggs activated with sperm were subjected to heat shock at 40 and 41 C for different postactivation times and durations. Diploid androgens had a normal appearance as controls and confirmed by chromosome counting ( n = 58). A maximum of 21 and 14% of diploidization was recorded at 30 min after activation, at 40 and 41 C, which corresponds to the first cleavage suppression time.
10 07 2014 10 2014 558 566 10.1111/jwas.12146 http://onlinelibrary.wiley.com/termsAndConditions#vor 10.1111/jwas.12146 https://onlinelibrary.wiley.com/doi/10.1111/jwas.12146 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jwas.12146 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjwas.12146 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjwas.12146 10.2331/suisan.58.1197 10.1016/0044-8486(95)01106-4 Bongers A. B. J. E. H.Eding andC. J. J.Richter.1993. Androgenesis in common carp Cyprinus carpioL. Proceedings of International Workshop on Genetics in Aquaculture and Fisheries Management Phuket November 1994. 10.1016/0044-8486(94)90504-5 10.1051/alr:1995035 10.1080/10454430902892941 10.1111/j.1365-2109.2010.02593.x 10.1186/2008-6970-4-4 10.1038/nrg1711 10.1002/jez.a.247 10.1002/jez.398 10.1577/1548-8659(1990)119<0698:PMAGIF>2.3.CO;2 10.1016/j.aquaculture.2003.12.016 10.1016/S0044-8486(03)00132-7 10.1139/g03-104 10.1139/f77-039 10.1016/j.aquaculture.2007.05.009 10.1016/0044-8486(91)90015-Y 10.1002/(SICI)1098-2795(199801)49:1<10::AID-MRD2>3.0.CO;2-R Recent advances in aquaculture McAndrew, B. J., 295 1993 10.1007/BF00222203 10.1007/s12562-010-0224-8 International Journal of Fisheries and Aquaculture Olufeagba, S. O. and 65 3 4 2011 Monosex production of African catfish, Heterobranchus longifilis, through gynogenesis and androgenesis Aquaculture Onozato, H. 97 43 91 1984 Diploidization of gynogenetically activated salmonid eggs using hydrostatic pressure 10.2331/suisan.49.693 10.1023/A:1003332526659 10.1038/hdy.1969.59 10.1016/0044-8486(86)90207-3 Fish physiology Thorgaard, G. H. 405 1983 10.1016/S0044-8486(96)01369-5