Development and Validation for the Simultaneous Quantification of Nebivolol Hydrochloride and Hydrochlorothiazide by UV Spectroscopy, RP‐HPLC and HPTLC in Tablets

Simultaneous quantification of nebivolol hydrochloride (NEB‐H) and hydrochlorothiazide (HCT) in tablets by UV spectroscopy, RP‐HPLC and HPTLC methods were developed. In UV spectrophotometric determination NEB‐H and HCT was quantified by simultaneous equation method and absorbance ratio method. In simultaneous equation method absorbance measurements at 282.5 nm (λ max NEB‐H) and 271.5 nm (λ max HCT), in absorbance ratio method absorbance measurements at 282.5 nm and 275 nm (iso absorptive point) in methanol. In RP‐HPLC method, the drugs were resolved using a mobile phase of 30 mM phosphate buffer (K 2 HPO 4 ), acetonitrile and triethylamine (50:50:0.1 % v/v) with pH 5.5 using orthophosphoric acid on a C 18 ‐ODS‐ Phenomenex (5 μm, 250 mm x 4.6 mm) column in isocratic mode, Atorvastatin (ATR) used as a internal standard. The retention time of HCT, NEB‐H and ATR was 3.31, 4.30 and 6.93 min respectively. In the HPTLC method, the chromatograms were developed using a mobile phase of ethyl acetate: methanol: ammonia (8.5:1:0.5 v/v) on precoated plate of silica gel 60 F 254 and quantified by densitometric absorbance mode at 285 nm. The Rf of HCT and NEB‐H were 0.21 and 0.41 respectively. Recovery studies of 98.88‐102.41%, percentage relative std deviation of not more than 0.8 and correlation coefficient (linearity range) of 0.9954‐0.9999 shows that developed methods were accurate and precise. These methods can be employed for the routine analysis of tablets containing NEB‐H and HCT.
08 20 2009 01 2010 341 348 10.1155/2010/483495 2 10.1002/crossmark_policy onlinelibrary.wiley.com true 2009-06-29 2009-08-20 2009-08-20 http://creativecommons.org/licenses/by/3.0/ 10.1155/2010/483495 https://onlinelibrary.wiley.com/doi/10.1155/2010/483495 http://downloads.hindawi.com/journals/jchem/2010/483495.pdf http://downloads.hindawi.com/journals/jchem/2010/483495.pdf http://downloads.hindawi.com/journals/jchem/2010/483495.pdf http://downloads.hindawi.com/journals/jchem/2010/483495.pdf http://downloads.hindawi.com/journals/jchem/2010/483495.pdf https://onlinelibrary.wiley.com/doi/pdf/10.1155/2010/483495 http://downloads.hindawi.com/journals/jchem/2010/483495.pdf