Concomitant Production, Partial Purification and Characterization of a Serine Protease and a Proteolysis-Resistant Metallolipase from Bacillus pumilus SG2

Concomitant Production, Partial Purification and Characterization of a Serine Protease and a Proteolysis-Resistant Metallolipase from Bacillus pumilus SG2 Ragupathy Sangeetha Department of Biochemistry, Vels University, Chennai – 600117, TN, India Arumugam Geetha Department of Biochemistry, Bharathi Women’s College, Chennai – 600108, TN, India. Irulandi Arulpandi Department of Microbiology, Asan Memorial College, Chennai – 600100, TN, India

Our objective was to investigate the concomitant production of protease and lipase by a bacterial strain. A promising bacterial strain was isolated from a food-processing industrial effl uent, which can produce both protease and lipase. The isolate was characterized by sequencing the16S rRNA gene. The PCR amplifi ed gene was subjected to analysis by BLAST to ascertain the genetic relatedness of the isolate, Bacillus pumilus SG2. The enzymes were produced and subjected to purifi cation by ammonium sulfate precipitation and dialysis followed by gel fi ltration chromatography; twelve-fold purity was obtained. The lipase produced was found to be proteolysis-resistant. The partially purifi ed enzymes were characterized for their optimum pH value, temperature, response to inhibitors, surfactants and oxidants. The relative molecular weights of protease and lipase were determined as 28 kDa and 40 kDa, respectively, by zymogram studies.
2 1 2010 6 2 2014 61 65 10.1515/znc-2010-1-211 http://creativecommons.org/licenses/by-nc-nd/3.0/ 10.1515/znc-2010-1-211 https://www.degruyter.com/document/doi/10.1515/znc-2010-1-211/html https://www.degruyter.com/document/doi/10.1515/znc-2010-1-211/pdf http://www.degruyter.com/view/j/znc.2010.65.issue-1-2/znc-2010-1-211/znc-2010-1-211.xml https://www.degruyter.com/document/doi/10.1515/znc-2010-1-211/xml