Direct observation of redox reactions in Candida parapsilosis ATCC 7330 by Confocal microscopic studies

Direct observation of redox reactions in Candida parapsilosis ATCC 7330 by Confocal microscopic studies Sowmyalakshmi Venkataraman Shoba Narayan Anju Chadha Abstract

Confocal microscopic studies with the resting cells of yeast, Candida parapsilosis ATCC 7330, a reportedly versatile biocatalyst for redox enzyme mediated preparation of optically pure secondary alcohols in high optical purities [enantiomeric excess (ee) up to >99%] and yields, revealed that the yeast cells had large vacuoles under the experimental conditions studied where the redox reaction takes place. A novel fluorescence method was developed using 1-(6-methoxynaphthalen-2-yl)ethanol to track the site of biotransformation within the cells. This alcohol, itself non-fluorescent, gets oxidized to produce a fluorescent ketone, 1-(6-methoxynaphthalen-2-yl)ethanone. Kinetic studies showed that the reaction occurs spontaneously and the products get released out of the cells in less time [5 mins]. The biotransformation was validated using HPLC.
10 14 2016 34344 BFsrep34344 1 10.1007/springer_crossmark_policy link.springer.com false 19 May 2016 13 September 2016 14 October 2016 The authors declare no competing financial interests. https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 10.1038/srep34344 20230105065742041 https://www.nature.com/articles/srep34344 https://www.nature.com/articles/srep34344.pdf https://www.nature.com/articles/srep34344.pdf https://www.nature.com/articles/srep34344 J. Ind. Microbiol. Biotechnol. T Kaliaperumal 37 159 2010 10.1007/s10295-009-0657-1 Kaliaperumal, T., Kumar, S., Gummadi, S. & Chadha, A. Asymmetric synthesis of (S)-ethyl-4-chloro-3-hydroxybutanoate using Candida parapsilosis ATCC 7330. J. Ind. Microbiol. Biotechnol. 37, 159–165 (2010). Tetrahedron: Asymmetry P Mahajabeen 22 2156 2011 10.1016/j.tetasy.2011.12.008 Mahajabeen, P. & Chadha, A. 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