IN VITRO PHARMACOLOGICAL SCREENING METHODS FOR ANTI-INFLAMMATORY AGENTS

IN VITRO PHARMACOLOGICAL SCREENING METHODS FOR ANTI-INFLAMMATORY AGENTS Jayakumari S Jijith Us

Context: The development of in vitro methods is important in drug discovery process due to the restriction of animal use in the pharmacological screening process.Objective: The objective of the review was to understand the in vitro pharmacological screening methods for anti-inflammatory agents.Methods: The present review details various in vitro methods available for the screening of activity of therapeutic agents against inflammation.Results: Inhibition and measurement of nitric oxide production, mast cell degranulation, leukocyte adhesion assay, platelet-neutrophil adhesion assay, lipopolysaccharide-induced response assay, N-formyl-methionyl-leucyl-phenylalanine-induced O2 generation by polymorphonuclear cells, and cyclooxygenase assays are discussed in this review.Conclusions: These methods help to understand the real mechanism of inflammation and mediators involved in this process and help toidentify new compounds possessing the anti-inflammatory activity.
12 28 2018 10.22159/ajpcr.2018.v11s4.31709 https://innovareacademics.in/journals/index.php/ajpcr/article/view/31709 https://innovareacademics.in/journals/index.php/ajpcr/article/viewFile/31709/16611 https://innovareacademics.in/journals/index.php/ajpcr/article/viewFile/31709/16611