Instrumental Analysis on Optimization of Laccase Production from Pleurotus ostreatus strain PKN 04 in Different Substrate by Solid State Fermentation (SSF)

Solid state fermentation (SSF) is a green process to produce enzymes from fungi. Different substrates such as rice husk, paddy straw, sugarcane bagasse and wheat husk taken from top soil were used to produce laccase by Pleurotus ostreatus strain PKN 04. The process of SSF was characterized by FTIR. Microbial fermentation resulted in conversion of complex polymeric lignocelluloses substrates into simpler monomers. Fourier transform infrared spectroscopy (FT-IR) analyses of metabolites confirmed the breakdown and complete degradation of lignin, cellulose and polysaccharides at different fermentation time intervals of 0, 15 and 30 days.