RP-HPLC Method for the Simultaneous Estimation and Validation of Amlodipine Besylate and Atenolol in Bulk and Tablet Dosage Form in Biorelevant Dissolution Medium (Fassif)

Objective: A simple, rapid, and precise reverse phase high performance liquid chromatographic (RP-HPLC)
method for simultaneous analysis of Amlodipine Besylate and Atenolol in a tablet dosage form and in
Biorelevant media has been developed and validated.
Methods:
The chromatographic separation was achieved using reverse phase C18 column; Kromasil C18 column (250 mm
x 4.6 mm x 5μm). The mobile phase used was a mixture of Acetonitrile: Potassium di hydrogen phosphate
solution (0.01M, pH 3.0 adjusting with Ortho phosphoric acid): Methanol (15:30:55) at isocratic mode and
eluents were monitored at 254 nm using PDA detector.