Oral Delivery of Curcumin Polymeric Nanoparticles Ameliorates CCl4-Induced Subacute Hepatotoxicity in Wistar Rats

Oral Delivery of Curcumin Polymeric Nanoparticles Ameliorates CCl4-Induced Subacute Hepatotoxicity in Wistar Rats Gregory Marslin Ratnam Institute of Pharmacy and Research, Nellore 524 346, India College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China http://orcid.org/0000-0002-2383-2382 Jose Prakash Department of Pharmaceutics, Jaya College of Paramedical Sciences, Tiruninravur 602 024, India Department of Pharmaceutics, Vels University, Chennai 600 117, India Shanshan Qi Dapartment of Pharmacology, Vitamin D research institute, Shaanxi University of Technology, Hanzhong 723000, China Gregory Franklin Department of Integrative Plant Biology, Institute of Plant Genetics of the Polish Academy of Sciences, 34 Strzeszynska Street, PL-60-479 Poznan, Poland http://orcid.org/0000-0002-6745-3528

Curcumin is the major bioactive compound of Curcuma longa, an important medicinal plant used in traditional herbal formulations since ancient times. In the present study, we report that curcumin nanoparticles (ηCur) protects Wistar rats against carbon tetrachloride (CCl4)-induced subacute hepatotoxicity. Nanoparticles of sizes less than 220 nm with spherical shape were prepared using PLGA and PVA respectively as polymer and stabilizer. Test animals were injected via intraperitoneal route with 1 mL/kg CCl4 (8% in olive oil) twice a week over a period of 8 weeks to induce hepatotoxicity. On the days following the CCl4 injection, test animals were orally administered with either curcumin or its equivalent dose of ηCur. Behavioural observation, biochemical analysis of serum and histopathological examination of liver of the experimental animals indicated that ηCur offer significantly higher hepatoprotection compared to curcumin.
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