CHITOSAN NANOPARTICLE ENCAPSULATED WITH Terminalia chebula – in vitro ANTIDIABETIC ACTIVITY

Terminalia chebula is an ayurveda herb which is used as the traditional medicinal
plant and provide an adverse effect in treating the disease. It is widely used in the
treatment of glucose- related diseases. The chitosan is a polymer that can be easily available in the form of biocompatible and non-toxic in nature. It can be used
as a biodegradable polymer and drug delivery in the pharmaceutical industry. The
detection of chitosan nanoparticle was carried out by characterisation methods like
UV-Vis. Spectroscopy, FE-SEM, EDS, FT-IR, XRD and DLS. Alpha amylase assay
and alpha glycosidase assay (glucose tolerance test) have been done for antidiabetic activity. The glucose metabolism and the viability of cells have been performed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Here the 3T3 fibroblastic cell line has been used. The glucose metabolism also confirms that the drug morphology and binding stability make the drug to treat the glucose-related diseases in absence of insulin. By the observation in future prospects, the chitosan-loaded nanoparticles have the potency on cancer therapy