Analytical method development and validation of glipizide by RP-UPLC method

A simple, precise, accurate method was developed for the estimation of Glipizide by RP-UPLC technique. Chromatographic conditions used are stationary phase BEH C18 (100mm x 2.1mm,1.8µm). Mobile phase composition of 0.01N KH2PO4: acetonitrile in the ratio of 50:50 and flow rate was maintained at 0.3ml/min, detection wave length was 234nm, column temperature was set to 30oC. System suitability parameters were studied by injecting standard six times and results were well under the acceptance criteria. Linearity study was carried out for 25% to 150% levels and R2 value was found to be 0.999. Precision was found to be 0.5 for repeatability and 0.3 for intermediate precision. LOD and LOQ are 0.05µg/ml and 0.14µg/ml respectively. By using above method, assay of marketed formulation was found to be 100.03%. Degradation studies of Glipizide were done in all conditions. Purity threshold was more than purity angle and lies within the acceptable range.