In vitro anti-prostate adenocarcinoma and lung cancer studies of phenoxyaniline- block -poly(methyl methacrylate) based nanocomposites via controlled radical polymerization

Phenoxyaniline-based macroinitiator is utilized for the first time in order to produce phenoxyaniline-lockedpoly(methyl methacrylate) composites through single electron transfer-living radical polymerization (SET-LRP) under mild conditions. A different weight percentage of Cloisite 93-A is added into the polymer mixtures in order to increase their biochemical properties. The prepared blocked copolymer nanocomposites are characterized using ATR-IR, UV-visspectroscopy, XRD, Raman, TGA, DSC, Particle size analyzer, contact angle measurement and SEM in order to characterize their structural, thermal, surface and morphological properties. Further, the developed polymeric nanocomposites are successfully applied in two different cancer cell lines (prostate adenocarcinoma and lung cancer), which show excellent anticancer properties. Also, the acridine orange/ ethidium bromide (AO\EtBr) dual staining is performed, which caused a
drastic cell death by apoptosis in both A549 and PC-3 cell lines, which indicated that the prepared polymeric
nanocomposites effectively inhibit the cell proliferation and induce the apoptosis in both the cancer cells. Here the
nanoclay is used for cancer treatment because of their complete water soluble in nature, which essentially forms a cationic complex between the clay and drug through electrostatic interactions. Hence, the exchange of ions between the clay and other ions in the biological environment is led to inhibit the proliferations of the prostate adenocarcinoma and lung cancer
cells in the system