Method Development and Validation of Famotidine Oral Suspension by RP-HPLC Method

For perseverance of Famotidine a simple, fast and selective procedure were
developed in drug substance and its pharmaceutical preparations. In the
proposed project, a successful attempt has been made to develop a simple,
accurate, economic and rapid method for the estimation and to validate the
method. As a result, a simple, economical, precise and accurate method was

developed and validated by Reverse Phase High Performance Liquid Chro-
matography (RP-HPLC). The main objective for that is to improve the con-ditions and parameers, which should be followed in the development andvalidation. The developed Reverse phase HPLC technique was done utilizingθiltered and degassed pH-6.0 Acetate buffer as a Mobile phase-A and pH-6.0Acetate buffer and organic mixture in the ratio of 30:70 as a Mobile phase-B. Byusing waters X-Bridge C18 150*4.6mm), 3.5μm column chromatographic sep-