Pre-analytical Errors in Glucose Estimation Results in Query on Diabetic Management

Abstract Status of diabetes of an individual is majorly
evaluated by the frequent monitoring of glucose estimation.

Use of serum samples and inappropriate plasma for esti-
mating glucose is an existing practise in Indian standard of

laboratories. There is a strong evidence for occurrence of
in vitro glycolysis on the above mentioned specimens. The
aim is to study the pre-analytical variations on the glucose
estimation of using sodium fluoride–disodium EDTA

(NaF–Na2EDTA) plasma (glycolysis inhibiting anticoag-
ulant) and determine the fact behind the activity of gly-
colysis inhibition on the same. Healthy volunteers

20–35 years of both genders consisting of 40 members
were selected for the study, and after getting the informed
consent form, random blood samples were collected to
study the errors of pre-analytical i.e., mixing of NaF–
Na2EDTA tube by phlebotomist (no of inversion). Dif-
ference in duration from blood collection to centrifugation
and a variable in time were taken from centrifugation to
analyzing the plasma sample. Comparative studies on
EDTA plasma and serum sample were also carried out. The