Screening for Anti-Diabetic Peptides from Moringa oleifera leaves

Screening for Anti-Diabetic Peptides from Moringa oleifera leaves Thiruchelvi. R Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai– 600 117, Tamil Nadu, India. Priyadharshini. S Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai– 600 117, Tamil Nadu, India. Rajakumari K. Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai– 600 117, Tamil Nadu, India.

Moringa oleifera commonly called as Drumstick tree is a multipurpose tree and it is widely present in the places like India, Asia, Africa, etc. Its leaves are emetic and their juices are used for medicinal purposes. Diabetes mellitus is a clinical syndrome with insufficient insulin secretion and abnormal glucose tolerance. It is a group of diseases which results in too much sugar in the blood. Leaves from Moringa oleifera were collected, analysed for their anti- diabetic activity. First the proteins are extracted using protein extraction methods, estimated using Lowry’s method, and then anti- diabetic activity was checked using Glucose uptake assay by yeast cells, where the plant extract (small proteins) was able to uptake glucose. SDS-PAGE was carried out to check the protein’s size and molecular weight. The characterization of Moringa oleifera conducted in this study shows that the small peptides from the leaves of this plant can contribute significantly in the daily recommended intake of it, since it serves as a rich source of all minerals, vitamins etc. This study has confirmed that the small peptides from the leaves of M.oleifera can fight against diabetics and the result indicated that Murungai (Moringa) leaves are suitable source of green leaf to reduce the diabetic complications in diabetic patients.
11 30 2021 11 30 2021 5886 5890 10.52711/0974-360X.2021.01023 https://rjptonline.org/AbstractView.aspx?PID=2021-14-11-49 Jangir RN, Jain GC. Antidiabetic and antioxidant potential of hydroalcoholic extract of Moringa oleifera leaves in streptozotocin-induced diabetic rats. European Journal of Pharmaceutical and Medical Research. 2016; 3:438-50. 10.3389/fphar.2012.00024 Mbikay M. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: a review. Frontiers in pharmacology. 2012 Mar 1; 3:24. 10.5897/ajb10.1599 Moyo B, Masika PJ, Hugo A, Muchenje V. Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. African Journal of Biotechnology. 2011;10(60):12925-33. 10.1017/s1751731107000298 Makkar HP, Francis G, Becker K. Bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems. animal. 2007 Oct;1(9):1371-91. 10.1155/2015/381040 Al-Malki AL, El Rabey HA. The antidiabetic effect of low doses of Moringa oleifera Lam. seeds on streptozotocin induced diabetes and diabetic nephropathy in male rats. BioMed research international. 2015;2015 10.4103/0970-5333.132846 Bhattacharya A, Agrawal D, Sahu PK, Kumar S, Mishra SS, Patnaik S. Analgesic effect of ethanolic leaf extract of Moringa oleifera on albino mice. Indian Journal of Pain. 2014 May 1;28(2):89. 10.4103/0973-8258.41182 Sutar NG, Bonde CG, Patil VV, Narkhede SB, Patil AP, Kakade RT. Analgesic activity of seeds of Moringa oleifera Lam. International Journal of Green Pharmacy (IJGP). 2008;2(2). 10.1016/0378-8741(88)90230-9 Shukla S, Mathur R, Prakash AO. Antifertility profile of the aqueous extract of Moringa oleifera roots. Journal of Ethnopharmacology. 1988 Jan 1;22(1):51-62. 10.1111/j.1753-0407.2011.00173.x Gupta R, Mathur M, Bajaj VK, Katariya P, Yadav S, Kamal R, Gupta RS. Evaluation of antidiabetic and antioxidant activity of Moringa oleifera in experimental diabetes. Journal of diabetes. 2012 Jun;4(2):164-71. 10.5923/j.microbiology.20120202.01 Abalaka ME, Daniyan SY, Oyeleke SB, Adeyemo SO. The antibacterial evaluation of Moringa oleifera leaf extracts on selected bacterial pathogens. Journal of Microbiology Research. 2012;2(2):1-4. 10.1002/ptr.2650090618 Pal SK, Mukherjee PK, Saha BP. Studies on the antiulcer activity of Moringa oleifera leaf extract on gastric ulcer models in rats. Phyto therapy research. 1995 Sep;9(6):463-5. Kalpana S, Moorthi S, Kumara S. Antimicrobial activity of different extracts of leaf of Moringa oleifera (Lam) against gram positive and gram-negative bacteria. International Journal of Current Microbiology and Applied Sciences. 2013;2(12):514-8. Patel P, Patel N, Patel D, Desai S, Meshram D. Phytochemical analysis and antifungal activity of Moringa oleifera. International Journal of Pharmacy and Pharmaceutical Sciences. 2014;6(5):144-7. 10.1186/1472-6882-13-226 Tiloke C, Phulukdaree A, Chuturgoon AA. The antiproliferative effect of Moringa oleifera crude aqueous leaf extract on cancerous human alveolar epithelial cells. BMC complementary and alternative medicine. 2013 Dec 1;13(1):226. Dahot MU. Antimicrobial activity of small protein of Moringa oleifera leaves. Journal of the Islamic Academy of Sciences. 1998;11(1):6. 10.4025/actascitechnol.v34i3.9605 Madrona GS, Branco IG, Seolin VJ, de Abreu Alves Filho B, Fagundes-Klen MR, Bergamasco R. Evaluation of extracts of Moringa oleifera Lam seeds obtained with NaCl and their effects on water treatment. Acta Scientia rum. Technology. 2012;34(3):289-93. 10.1016/s0021-9258(19)52451-6 Lowry OH, Rosebraugh NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of biological chemistry. 1951; 193:265-75. 10.3389/fphar.2017.00577 Khan W, Parveen R, Chester K, Parveen S, Ahmad S. Hypoglycaemic potential of aqueous extract of Moringa oleifera leaf and in vivo GC-MS metabolomics. Frontiers in pharmacology. 2017 Sep 12; 8:577. 10.1515/jbcpp-2014-0129 Olayaki LA, Irekpita JE, Yakubu MT, Ojo OO. Methanolic extract of Moringa oleifera leaves improves glucose tolerance, glycogen synthesis and lipid metabolism in alloxan-induced diabetic rats. Journal of basic and clinical physiology and pharmacology. 2015 Nov 1;26(6):585-93. 10.12688/f1000research.15642.1 Banik S, Biswas S, Karmakar S. Extraction, purification, and activity of protease from the leaves of Moringa oleifera. F1000Research. 2018;7. 10.3390/molecules22020271 Paula PC, Sousa DO, Oliveira JT, Carvalho AF, Alves BG, Pereira ML, Farias DF, Viana MP, Santos FA, Morais TC, Vasconcelos IM. A protein isolate from Moringa oleifera leaves has hypoglycaemic and antioxidant effects in alloxan-induced diabetic mice. Molecules. 2017 Feb;22(2): 271.Tables