Phytochemical Analysis by HR-LCMS and <i>In vitro</i> Anti-diabetic Potential of <i>Michelia champaca</i> Bark

Phytochemical Analysis by HR-LCMS and <i>In vitro</i> Anti-diabetic Potential of <i>Michelia champaca</i> Bark Segu Prathyusha Malarkodi Velraj

The current analysis was aimed to study the phytochemical profile and in vitro antidiabetic capacity of HEMC bark. HR-LCMS1 was used to identify the phytochemicals present in the extract. The outcomes of HR-LCMS showed the presence of 15 phytochemical compounds. DL-Carnitine, Catechin, D-β-Tocopherol, Colchicine, Myricetin, Epicatechin, Quercetin, Epigallocatechin gallate, Quercetin-3β-D-glucoside, Kaempferol, Sorbic acid, Apocynin, Epigallocatechin gallate, myricetin 3-O-beta-D-galactopyranoside, Naringeninchalcone are the main compounds identified. The inhibition of enzymes like β-amylase and β-glucosidase delays the rate of glucose absorption thus reducing blood glucose levels in the experimental models. The IC50 values of β-amylase and β-glucosidase inhibitory activity of HEMC were acquired to be 88.65 ?g/mL and 71.28 ?g/mL correspondingly. Positive control acarbose displayed IC50 assessment of 52.94 ?g/mL and 50.01 ?g/mL correspondingly. Consequently, the current study confirms that HEMC had remarkable antidiabetic activity and hence holds future potential as nutraceuticals in the treatment of diabetes and related ailments.
07 30 2022 432 439 10.18311/jnr/2022/29798 https://informaticsjournals.com/index.php/jnr/article/view/29798 https://informaticsjournals.com/index.php/jnr/article/download/29798/21289 https://informaticsjournals.com/index.php/jnr/article/download/29798/21289 Digambar Subhashrao Pawar and Sahera Nasreen, HR-LCMS of phytoconstituents and antifungal activity of medicinal plants, Journal of Medicinal Plants Studies 2018; 6(1): 173-176 10.1007/978-3-642-00574-9 Wagner H, Bladt S. Plant drug analysis- a thin layer chromatographic Atlas, Springer, Munich, Germany, 1996, Pp. 106-134. Harbone JB. Phytochemical methods- A guide to modern techniques of plant analysis. 3rd Ed, Springer, Rajkamal Electronic printing press, New Delhi, 1998, Pp. 283-299. Pulok KM. Quality control Botanicals- An approach to evaluation of botanicals, 2nd Ed, Business Horizons Pharmaceutical Publications, New Delhi, 2008, Pp. 153-169. Sangeetha S, Mary Shoba DC Gayathri DS. Comparative Evaluation of Free Radical Scavenging Activity of Cleome Viscose and Trichodesma indicum. International Journal of Pharmacy and Pharmaceutical Sciences 2014, 6(1), 318-325. 10.3923/ajsr.2012.1.13 Sujatha S, Shalin JJ. Complementary therapeutic potential: A focus on Polyherbal products for hyperglycemia. Asian J Sci Res 2012;5:1 13. 27. Srivastava S, Lal VK, Pant KK. Polyherbal formulations based on Indian medicinal plants as antidiabetic phytotherapeutics. Phytopharmacology 2012;2:1 15. 28. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 2001;50:536 46. 30. 10.22159/ijpps.2017v9i8.19411 Priyanka Jaiswal, Padma Kumar. Alpha amylase inhibitory activity of different extract of bark of Albizia lebbeck (L.) benth. Int J Pharm Pharm Sci 2017;9(8): 10. Abdollahi M, ZukiAB, GohYM, RezaeizadehA, Noordin MM. Effects of Momordica charantia on pancreatic histopathological changes associated with streptozotocin induced diabetes in neonatal rats. Histol Histopathol 2011;26:13 21 10.1016/S0168-8227(98)00043-6 Baron AD. Postprandial hyperglycaemia and alpha-glucosidase inhibitors. Diabetes Res Clin Pract. 1998;40(Suppl):S51–5. 10.1016/j.lwt.2011.08.006 Kunyanga CN, Imungi JK, Okoth MW, Biesalski HK, Vadivel V. Total phenolic content, antioxidant and antidiabetic properties of methanolic extract of raw and traditionally processed Kenyan indigenous food ingredients. LWT-Food Sci Technol 2012;45:269-76. Chitravadivu C, Manian S, Kalaichelvi. Qualitative Analysis of selected Medicinal Plants, Tamilnadu, India. Middle-East Journal of scientific Research. 2009; 4(3):144-146. Digambar Subhashrao Pawar and Sahera Nasreen, HR-LCMS of phytoconstituents and antifungal activity of medicinal plants, Journal of Medicinal Plants studies, JMPS 2018; 6(1): 173-176 10.3724/SP.J.1009.2010.00349 Ye X.P.; Song, C.Q.; Yuan, P.; Mao, R.G. β-Glucosidase and β-Amylase Inhibitory Activity of Common Constituents from Traditional Chinese Medicine Used for Diabetes Mellitus. Chin. J. Nat. Med. 2012, 8, 349?352.