Synergistic effect of plant extracts from delonix, hygrophila auriculata and drynaria quercifolia against inflammation

Inflammatory diseases including different types of rheumatics are a major cause of
morbidity worldwide.This study addresses the global burden ofinflammatory diseases,
focusing on safer alternatives to synthetic anti-inflammatory drugs. Extracts from ethnomedicinal plants, Delonix elata, Hygrophila auriculata, and Drynaria quercifolia,
were combined to enhance their anti-inflammatory properties. The ethanolic extract
was obtained from the leaves of Delonix elata, seeds of Hygrophila auriculata, and
rhizomes of Drynaria quercifolia, showing the existence of phenolics, flavonoids,
and alkaloids in preliminary phytochemical screening. Two combinations, D (1:1:1)
and E (10:5:1), were prepared. In vitro studies revealed that combination D shown
maximum total antioxidant activity the strongest capacity to scavenge DPPH radicals.
Antibacterial assays demonstrated varying zones of inhibition for E. coli and S. aureus
among the combinations. Notably, combination D showed the largest zone of inhibition
for E. coli, while sample C was most effective against S. aureus. Protein denaturation assay for anti-inflammatory activity highlighted combination D's remarkable inhibition of BSA denaturation (97.37% at 1mg/ml concentration). This research explores
potential natural remedies and holistic healthcare solutions through the synergistic
effects of plant extracts, aligning with traditional medicinal knowledge.