Anticancer Potential of Zeatin in HepG2 Liver Cancer Cell Line

Liver cancer remains a major global health challenge, necessitating the exploration of novel therapeutic
agents with potent anticancer activity. Zeatin, a naturally occurring cytokinin, has demonstrated diverse
biological properties, including antioxidant and anti-inflammatory effects. This study evaluates the
biochemical properties and anticancer potential of zeatin against human hepatocellular carcinoma
(HepG2) cells. Zeatin’s cytotoxic effects were assessed using the MTT assay, while its impact on apoptosis
was investigated through AO/EB and PI staining. Additionally, biochemical assays were performed to
determine its influence on oxidative stress markers and key apoptotic regulators. Results revealed a dosedependent
reduction in HepG2 cell viability, along with enhanced apoptosis induction and alterations in
oxidative stress parameters. These findings suggest that zeatin exerts significant anticancer activity
against liver cancer cells, potentially through apoptotic and oxidative stress-mediated pathways. Further
molecular studies are warranted to elucidate its precise mechanism of action and therapeutic
applicability.