Formulation and Development of Bioactive Electrospun Nanofibers for Regenerative Medicine: Egg Yolk Oil and Rosehip Seed Oil Novel Biomaterial-Based Approaches

This study reports the formulation and evaluation of bioactive electrospun nanofibers incorporating egg yolk oil (EYO)
and rosehip seed oil (RSO) within polycaprolactone–gelatin scaffolds for regenerative medicine. Optimized nanofibers
displayed uniform morphology, high encapsulation efficiency (>85%), and sustained release profiles exceeding 90% within
48 hours. FTIR and DSC confirmed compatibility of oils with polymers, while SEM revealed bead-free fiber structures.
Mechanical testing indicated sufficient tensile strength for scaffold stability. In vitro assays demonstrated excellent
cytocompatibility, with nanofiber-treated groups showing >95% cell viability compared to controls. Anti-apoptotic studies
confirmed enhanced Bcl-2 expression and reduced cleaved caspase-3, indicating suppression of apoptosis. ELISA further
validated upregulation of antioxidant and regenerative markers. Compared to synthetic scaffolds, EYO-based nanofibers
provided superior cell adhesion, metabolic activity, and faster release kinetics, whereas RSO-based scaffolds offered
prolonged bioactivity. Collectively, these results suggest that natural oil-infused nanofibers can synergize with polymers to
create bioactive, biocompatible scaffolds suitable for wound healing, bone regeneration, and broader regenerative
applications. Future work should address long-term stability, large-scale production, and regulatory compliance to facilitate
clinical translation...