Biosynthesis of Silver Nanoparticles Using Cinnamomum tamala Leaf Extract and Revealing Its Antioxidant Activity

Silver nanoparticles (AgNPs) possess distinctive properties. This study examines the biosynthesis of AgNPs with antioxidant
properties using Cinnamomum tamala leaves through green chemistry. Silver nitrate (AgNO3) was converted to AgNPs
mediated Cinnamomum tamala leaf extract method, providing a cost-effective and eco-friendly alternative to conventional
methods. X-ray diffraction (XRD) confirmed the crystalline structure of AgNPs with an average size of 21.92 nm. Scanning
electron microscopy (SEM) and Transmission electron microscopy (TEM) analyzed the nanoparticles' morphology and size
distribution. The AgNPs were found to range from 30 to 60 nm, establishing their sub-100 nm size. The presence of AgNPs
was verified by a peak at 3-3.5 keV in the EDX spectrum. Ultraviolet-visible (UV-Vis) spectroscopy and Fourier-transform
infrared spectroscopy (FTIR) were employed to examine the reduction of AgNPs and stable phytochemicals. Various radical
scavenging activities were evaluated to determine antioxidant capacity. The antioxidant activity was assessed through DPPH,
superoxide anion, hydroxyl, hydrogen peroxide, and nitric oxide scavenging assays. AgNPs exhibited substantial antioxidant
activity with IC50 values of 58.88μg/mL, 57.18μg/mL, 53.58μg/mL, 61.50μg/mL, and 56.02μg/mL respectively, indicating
effective free radical scavenging. The results highlight the significant antioxidant properties of both the leaf extract and
AgNPs. Synthesized AgNPs exhibited enhanced characteristics compared to the leaf extract. AgNPs from Cinnamomum
tamala leaf extract show considerable potential as antioxidants for nutraceutical and pharmaceutical applications against
oxidative stress-related diseases.