In Vitro Antioxidant Potential of Aqueous Extract of Annona Muricata Bark

The in vitro antioxidant potential of aqueous extract of Annona muricata bark was evaluated using a series of well-established assays. The extract demonstrated remarkable antioxidant activity, assessed through the ABTS, lipid peroxidation inhibition, superoxide radical scavenging, nitric oxide radical scavenging, and metal chelating assays. The ABTS assay revealed a strong radical scavenging activity, with a notable EC50 value, indicating its capacity to neutralize reactive oxygen species. Similarly, lipid peroxidation activity was significant, with the aqueous extract of Annona muricata displaying a considerable reduction in lipid peroxidation, with an EC50 value comparable to that of standard ascorbic acid. The superoxide radical scavenging activity also exhibited promising results, showing substantial inhibitory effects on superoxide radicals. Furthermore, nitric oxide scavenging activity demonstrated an EC50 value close to the standard, reflecting the extract’s potential to mitigate nitric oxide-induced cellular damage. The metal chelating activity of the extract was also evaluated, with the aqueous extract showing a significant capacity to bind metal ions, a crucial mechanism in antioxidant defense. These findings suggest that Annona muricata bark extract holds substantial promise as a natural antioxidant, capable of mitigating oxidative stress through multiple mechanisms. This study supports the potential application of Annona muricata as a source of bioactive compounds for therapeutic use, particularly in the prevention of oxidative stress-related diseases.