Determination of Bioactive Compounds Using GC-MS Analysis And Evaluation of Antioxidant Activity of Catharanthus Roseus Leaves Extract

The aim of the study was to assess the different phytochemical profiles and the GC-MS analysis of the extracts of Catharanthus roseus leaves. The whole extract of Catharanthus roseus leaves showed the presence of tannin, saponin, steroids, terpenoids, alkaloids, flavonoids, triterpenoids, polyphenol, glycoside and coumarins, while anthocyanins were missing. Anthroquinone was contained in the ethanol and hydroethanol extract. The robe Catharanthus leaf extract had a significant concentration of polyphenol, flavonoid, tannin and terpenoids. GC-MS analysis of the leaves of Catharanthus roseus showed the presence of 20 compounds. The compounds identified have many biological properties. Antioxidant activity sieved by DPPH, total antioxidant activity, hydrogen peroxide, ABTS (2.2'-azino-bis- 3 -ethylbenzthiazolin- 6 -sulfonic acid) and nitrogen oxide scavenging activity at various concentrations and ascorbic acid as standard antioxidant.