In vitro anti-prostate adenocarcinoma and lung cancer studies of phenoxyaniline- block -poly(methyl methacrylate) based nanocomposites via controlled radical polymerization

In vitro anti-prostate adenocarcinoma and lung cancer studies of phenoxyaniline- block -poly(methyl methacrylate) based nanocomposites via controlled radical polymerization Sahariya Priya School of Chemical Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk 38541, Republic of Korea http://orcid.org/0000-0003-2410-3605 Adhigan Murali School for Advanced Research in Petrochemicals (SARP)- ARSTPS, Central Institute of Petrochemicals Engineering & Technology (CIPET), Govt. of India, Chennai, 600032, India http://orcid.org/0000-0003-2726-8085 Sakar Mohan Centre for Nano and Material Sciences, Jain University, Bangalore 562112, Karnataka, India A. Lakshminarayanan Department of Pharmacology, Indira Medical College and Hospitals, Tiruvallur, Tamilnadu, 631 203, India S. Sekar School for Advanced Research in Petrochemicals (SARP)- ARSTPS, Central Institute of Petrochemicals Engineering & Technology (CIPET), Govt. of India, Chennai, 600032, India R. Ramesh Department of Chemical Engineering, School of Mechanical, Chemical and Material Engineering, Adama Science and Technology University, Adama, P.O. Box: 1888, Adama, Ethiopia http://orcid.org/0000-0002-1008-5859 M. Devendiran Vels Institute of Science Technology and Advanced Studies (VISTAS), Pallavaram, Chennai 117, India http://orcid.org/0000-0001-6412-6006 Sung Soo Han School of Chemical Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk 38541, Republic of Korea

A phenoxyaniline-based macroinitiator is utilized for the first time in order to produce phenoxyaniline- block -poly(methyl methacrylate) composites through single electron transfer-living radical polymerization (SET-LRP) under mild conditions.
A phenoxyaniline-based macroinitiator is utilized for the first time in order to produce phenoxyaniline- block -poly(methyl methacrylate) composites through single electron transfer-living radical polymerization (SET-LRP) under mild conditions. A different weight percentage of Cloisite 93A is added into the polymer mixtures in order to increase their biochemical properties. The prepared block copolymer nanocomposites are characterized using ATR-IR, UV-vis-spectroscopy, XRD, Raman, TGA, DSC, a particle size analyzer, contact angle measurements and SEM in order to characterize their structural, thermal, surface and morphological properties. Further, the developed polymeric nanocomposites are successfully applied in two different cancer cell lines (prostate adenocarcinoma and lung cancer), which show excellent anticancer properties. Also, acridine orange/ethidium bromide (AO/EtBr) dual staining is performed, which causes drastic cell death by apoptosis in both A549 and PC-3 cell lines, which indicated that the prepared polymeric nanocomposites effectively inhibit the cell proliferation and induce the apoptosis in both the cancer cells. Here nanoclay is used for cancer treatment because of its complete water solubility, which essentially causes the formation of a cationic complex between the clay and drug through electrostatic interactions. Hence, the exchange of ions between the clay and other ions in the biological environment leads to inhibition of the proliferation of prostate adenocarcinoma and lung cancer cells in the system.
2023 5870 5879 1 10.1039/rsc_crossmark_policy rsc.org true National Research Foundation of Korea https://doi.org/10.13039/501100003725 2020R1A6A1A03044512 Ministry of Agriculture, Food and Rural Affairs https://doi.org/10.13039/501100003624 321027-5 http://creativecommons.org/licenses/by-nc/3.0/ 10.1039/D3NA00644A 20231024184233 https://xlink.rsc.org/?DOI=D3NA00644A http://pubs.rsc.org/en/content/articlepdf/2023/NA/D3NA00644A ACS Nano Kim 8 2014 10.1021/nn503349g 9358 Int. J. Oncol. Liu 45 2014 10.3892/ijo.2014.2561 1735 Inorg. Chim. Acta Kumar 538 2022 10.1016/j.ica.2022.120963 120963 Clay Miner. Hosseini 53 2018 10.1180/clm.2018.4 53 Bull. Mater. Sci. Patel 29 2006 10.1007/BF02704606 133 Macromol. Chem. Phys. Sparnacci 203 2002 10.1002/1521-3935(200207)203:10/11<1364::AID-MACP1364>3.0.CO;2-6 1364 Drug Delivery Zobel 7 1997 483 J. Appl. Polym. Sci. Rao 111 2009 10.1002/app.29057 845 Polym. Chem. Geyik 6 2015 10.1039/C5PY00780A 5470 Nanomedicine Seyedeh 6 2010 10.1016/j.nano.2010.01.011 689 Clin. Nutr. Cury-Boaventura 23 2004 10.1016/j.clnu.2003.12.004 721 J. Mater. Sci.: Mater. Electron. Li 31 2020 2708 Int. J. Electrochem. Sci. Mathew 2015 1 J. Raman Spectrosc. Mingming 51 2020 10.1002/jrs.5754 115 J. Polym. Res. Arora 18 2011 10.1007/s10965-010-9481-6 843 Int. J. Nanomed. Elhussein 9 2014 3771 Lasers Med. Sci. Manoto 26 2011 10.1007/s10103-011-0887-0 523 J. Oral Pathol. Med. Ezhilarasan 48 2019 10.1111/jop.12806 115 Pharmacogn. J. Usmani 6 2014 10.5530/pj.2014.6.7 32 Trans. Nonferrous Met. Soc. China Suresh 24 2014 10.1016/S1003-6326(14)63412-9 2805 Food Chem. Toxicol. Sathivel 46 2008 10.1016/j.fct.2008.07.016 3262 Clin. Oral Investig. Govindaraju 2017 10.1007/s00784-016-1922-0 567 J. Mater. Res. Technol. Sathish 9 2020 10.1016/j.jmrt.2020.01.085 3481 Therm. Sci. Krishnaswamy 2020 10.2298/TSCI190409397K 495