Affinity Purification Lipidomics

Lipidomics is a growing area of study that aims to understand the complex structure of lipid molecules in living organisms, relying on strong techniques to separate and study certain lipid types. Affinity purification is a crucial technique in lipidomics that allows for the selective extraction of lipids based on their distinct features. This chapter thoroughly examines the fundamentals, applications, and current breakthroughs of affinity purification in the field of lipidomics. The chapter discusses affinity tags and ligands designed for certain lipid components, highlighting their importance in improving the accuracy and effectiveness of lipid extraction. Affinity purification is used to study lipid–protein interactions, analyze lipid rafts, and understand the various functions of lipids in biological processes. This method provides valuable insights into these specific areas. The chapter discusses current breakthroughs in affinity purification techniques designed for lipidomics, focusing on improvements in matrix design and the creation of high-throughput approaches. The developments aim to overcome the difficulties in lipidomics research by focusing on isolating low-abundance lipid species and selectively enriching bioactive lipids. Finally, this chapter provides a valuable guidance for incorporating affinity purification into lipidomics techniques.