A review of the development of analytical methods by RP-HPLC for vitamin D3

An analytical approach for quantifying vitamin D3
was developed using high-performance liquid
chromatography (HPLC). The vitamin D3
was separated using a C-18 column. In the mobile phase, a
methanol-to-water ratio of 97:3 was used. The wavelength of the vitamin D3
chromatogram was 264 nm,
and the flow rate was 1.2 ml/min. In this study, analytical quality by design (AQbD) approaches were used
to optimize chromatographic parameters for routine cholecalciferol (CHL) assays. Key technique
parameters were screened and optimized using Taguchi orthogonal array design and Box-Behnken design
to enhance the approach's performance. Vitamin D3
insufficiency can lead to illnesses and lowered
immunity, if one lives in a northern climate and eats an insufficient diet. To compensate for vitamin D
insufficiency, prescription drugs that include cholecalciferol, often known as vitamin D3
, one of the active
forms of vitamin D, are utilized. Make an effort to determine the cholecalciferol content of certain
pharmaceuticals and dietary supplements that are sold in the Russian Federation, as well as to develop and
validate an HPLC technique for detecting vitamin D3
in vitamin drugs. The robustness of the method was
demonstrated by the fact that the findings did not significantly change when the mobile phase concentration
and flow rate were adjusted. The proposed method is suitable for bulk vitamin D3
measurement in a variety
of pharmaceutical formulations and passed all validation tests.