Analysis of phytoconstituents and its phytoformulation of curcumin chewable tablet as per ICH guidelinesm

A simple, selective, precise RP-HPLC method has been developed for the determination of curcumin in
pharmaceutical formulation. Curcumin was analyzed by HPLC using C18 analytical column (250 mm x 4.6
mm, 5 µm) with UV detection at 420 nm. The mobile phase consisted of a mixture of 60 volumes of
tetrahydrofuran and 40 volumes of citric acid. The chromatography was performed by isocratic elution
at a flow rate of 1.0 ml/min. The detector response for the curcumin was linear over the selected
concentration range from 1 to 10 µg/ml, with a correlation coefficient of 0.9999. The accuracy was
between 98.0% to 102.0%. The precision (R.S.D) among the six sample preparations was 0.9%. The
recovery of curcumin was about 100.92%. The method was validated according to International Conference
of Harmonization Guidelines in terms of accuracy, precision, linearity, specificity, robustness, ruggedness,
and solution stability method. The method development of curcumin was applied successfully for HPLC
analysis of pharmaceutical dosage form.