High-Performance Thin Layer Chromatography (HPTLC) Analysis for the Quantification of Constituents in Ficus religiosa

has drawn scientific interest due to its diverse chemical composition. This study aimed to
develop a densitometric High-Performance Thin Layer Chromatography (HPTLC) technique
to estimate phenolic compounds present in the hydroalcoholic extract of dried root bark of
Ficus religiosa. Materials and Methods: This study utilized HPTLC to identify the constituents
within Ficus religiosa root bark. The isolated constituent was evaluated by various analytical
methods like phytochemical tests, UV, HPTLC etc. Subsequently, the HPTLC method underwent
rigorous validation, confirming its reliability and accuracy in separating and quantifying the
constituents within the root bark of Ficus religiosa. Results: Isolation of phytoconstituents was
done by column chromatographic technique using toluene: ethyl acetate: methanol (3:3:2).
The isolated component was confirmed as a flavonoid moiety by various chemical tests and by
analytical evaluation. Also, HPTLC analysis of the isolate revealed the compound was Quercetin a
flavonoid moiety or its derivative. Conclusion: These findings underscore the presence of main
phytoconstituents in Ficus religiosa root bark which is responsible for pharmacological effects
and offers insights into its application in both traditional and contemporary medicinal practices.