Fabrication and Cytotoxic activity of Clofarabine Nano Emulzion Decorated with Chitosan on MCF-7 cell linings

Fabrication and Cytotoxic activity of Clofarabine Nano Emulzion Decorated with Chitosan on MCF-7 cell linings Annapurna Anusha Kotwala Department of Pharmaceutics, School of Pharmaceutical Sciences, Vels Institute of Science Technology and Advanced Studies (VISTAS), Chennai - 600117, Tamilnadu, India. Komala. M Department of Pharmaceutics, School of Pharmaceutical Sciences, Vels Institute of Science Technology and Advanced Studies (VISTAS), Chennai - 600117, Tamilnadu, India.

One antineoplastic analogue of purine nucleosides from the second generation is clofarabine. This study aimed to produce clofarabine-loaded chitosan nanoparticles as a possible drug delivery vehicle for anticancer11,12. The design and development of innovative drug delivery systems, mostly based on nanotechnology, has become necessary due to the constraints associated with the conventional treatment of cancer. Chitosan-based nanoparticles have caught the interest of researchers as one of the many innovative medication delivery strategies for the treatment of cancer. Chitosan is a polycationic polymer that has several properties, including biocompatibility, biodegradability, and non-toxicity, which makes it the perfect polymer for creating drug delivery systems. Chitosan was used to cover the prepared nano-emulzion of CLOFARABINE. A two-step emulsification procedure was used in the current investigation to create chitosan-decorated multiple Nano emulzions (MNEs). A total of five MNE sample formulations were created and assessed. Entrapment efficiency (EE), stability, zeta potential, globule size, PDI and invitro drug release tests were used to characterize the produced formulations. The manufactured MNE displayed regulated drug release. The chitosan ornamentation used to create sustained release for the MNE. Additionally, studies were conducted on conjugated nanoparticles' cytotoxicity.
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