Polyherbal Hand Hygiene Solutions: Formulation Optimization and Broad-Spectrum Antimicrobial Activity

In recent pandemic condition, the personal hygiene is considerable as vital
factor more over it was recommended by WHO to protect us from life threatening Covid
19. Many companies formulated sanitizer using alcohol and other chemicals. With or
without our knowledge we used them in excess to protect ourselves from dreadful
condition. Most of the sanitizer were found to unwanted effect on the skin, allergic, causes
environmental pollution and cost effective. So, we decided to formulate the sanitizer purely
based on herbs without any chemical or artificial colour and perfumes. An attempt was
made to formulate and evaluate herbal hand sanitizer and herbal hand wash based
available on earlier claims of our indigenous plants. The formulations were evaluated for
PH, stability test, organoleptic test and preliminary chemical test. The evaluation results
were found to be within the standard limit. The presence of poly phenolic compounds,
steroids, alkaloids and tannins were found by chemical test. Both the formulations were
tested for antimicrobial activity with bacterial species and fungal species. The selected
bacterial species are bacillus subtitles (MTCC 6133), Escherichia coli (MTCC 1687) &
pseudomonas aeruginosa (MTCC 7925) and fungal species. Hand sanitizer showed the very
good suitability effect on the both fungal species and bacterial species. Hand wash shows
the good sustainability effect on the bacterial species whereas the poor effect on the fungal
species. Comparatively hand sanitizer shows the good antibacterial activity as well as
antifungal activity against tested organism by agar well diffusion method. It shows that out
of two formulations, hand sanitiser formula was found as good antimicrobial agent. The
results of study was very encouraging to us.