FROM METHOD DEVELOPMENT TO VALIDATION: RP-HPLC STRATEGIES FOR LENACAPAVIR AND ITS COMBINATIONS- A REVIEW

Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) remains a cornerstone technique in pharmaceutical analysis for antiviral agents special emphasises on lenacapavir and its combinations. This review summarizes recent trends (past ~5 years) in method development and validation by RP-HPLC for antiviral drugs, covering column technologies, mobile phase strategies, detection options, sample preparation, automation, and regulatory-aligned validation practices. Special focus is given to multi-analyte methods, hyphenated techniques, green analytical chemistry, and challenges in sensitivity and matrix complexity. The article concludes with future directions to guide analysts and quality control laboratories.