Silver Nanoparticles and Its Invitro Cytotoxic Behaviour- A Fungi Aided Synthesis

Nanotechnology is concerned with the creation and stabilisation of nanoparticles. The
biological method necessitates the creation of nanoparticles that are eaten by microorganisms
capable of digesting nanoparticles in various forms. The fungus Pestaloptiopsis breviseta is
used in this study to demonstrate the extracellular production of stable silver nanoparticles.
The fungal culture was isolated from a stable Catharanthus roseus (L) G.don leaf sample, a
common therapeutic plant. They were produced after the AgNO3 solution was employed to
treat the cell filtrate and the fungal mat at room temperature and in the dark. (1 mM). The cell
filtrate made silver nanoparticles that were between 171-378 nm in size, whereas the fungal
biomass was between 140-280 nm in size. The cell lines MCF-7 and A549 were likewise treated
with the silver nanoparticles made by the fungi. GraphPad Prism 5 software was used to track
the percentage of living cells for 24 and 48 hours at different concentrations of the MCF-7 and
A549 cell lines based on the IC50 value.