DESIGN AND NOVEL SYNTHESIS OF SCHIFF BASE DERIVATIVE FOR ANTI INFLAMMATORY ACTIVITY

This research focuses on the design, synthesis, and evaluation of coumarin Schiff
basederivatives as potential anti-inflammatory agents with fewer side effects than traditional
non-steroidal anti-inflammatory drugs (NSAIDs). NSAIDs are widely used but are
associatedwith gastrointestinal and cardiovascular toxicity, creating a need for safer
alternatives. In this study, four Coumarin-based compounds were designed and
synthesized.Drug-likeness of the compounds was assessed using Lipinski’s Rule of Five,
confirming their suitability for oral use. Molecular docking studies were performed against
thecyclooxygenase-2 (COX-2) enzyme to predict binding affinity and interaction patterns.
All compounds showed better binding energy compared to the standard drug
ibuprofen,indicating strong potential for anti-inflammatory activity. The synthesized
compounds were further evaluated through an in vitro protein denaturation assay to measure
anti-inflammatory effects. revealed that compounds 6 and 7 exhibited higher inhibition of
protein denaturation than ibuprofen. Among them, compound 7 demonstrated the best overall
performance, with the highest inhibition percentage and strong statistical correlation between
concentration and activity. Although compound 8 showed excellent docking , its potential
gastrointestinal side effects reduce its suitability as a lead compound. Overall, the study
concludes that compound 7 is the most promising candidate for further development. Future
research should focus on toxicity studies and clinical evaluation to confirm its safety and
effectiveness.