Exploring antibiotic degradation mechanisms: Molecular docking analysis of beta-lactamase enzymes from Pseudomonas songnenensis

This study investigates the potential of Pseudomonas songnenensis (P. songnenensis) in degrading β-lactam anti
biotics through enzymatic hydrolysis by β-lactamase. Faecal soil samples were collected from ten poultry farms in
Tamil Nadu, India, between June and July 2023. Each farm houses 10,000–50,000 birds and routinely admin
isters antibiotics. Among the bacterial isolates obtained, strain 18 showed the highest degradation activity.
Molecular docking analysis revealed stable enzyme-antibiotic interactions, with Amoxicillin showing the
strongest binding affinity due to multiple hydrogen bonds. The β-lactamase enzyme effectively hydrolyses the
β-lactam ring, breaking the amide bond and rendering antibiotics inactive. This stepwise degradation mechanism
contributes to reducing antibiotic persistence in the environment and offers insights into microbial-driven
bioremediation strategies. The findings highlight the novelty of using P. songnenensis for antibiotic degrada
tion and emphasise its potential application in mitigating antibiotic pollution in livestock farming and food
production systems.