Green Synthesis of Silver Nanoparticles using Zanthoxylum armatum Seed Extract and Its Characterization, Efficacy on Antioxidant and Antibacterial Properties

Silver nanoparticles are nanosized particle which has antibacterial and antioxidant activity. The present study
highlighted a biocompatible and eco-friendly method for the synthesis of nanoparticles through green
synthesis using Zanthoxylum armatum seed extract. The synthesized nanoparticle was analyzed through
analytical techniques. Physically, the presence of AgNPs was confirmed by color change from pale yellow to
yellowish-brown color. UV-Visible spectroscopy revealed a peak at 332nm. And the FTIR showed the presence
of N-H stretch, O-H stretch, and CN stretch, confirming the phytochemicals reduction and presence of
Alkaloids, flavonoids, and Polyphenols. The DLS depicted low PDI values, which indicated the presence of
moderate polydispersity. The in vitro antibacterial activity showed the potential of ZAAgNps against bacteria
like Klebsiella pneumoniae, S. aureus, and E. coli, with zone of inhibition 19mm, 16mm, and 14mm at their highest
concentrations. The antioxidant potency of ZAAgNps was estimated by in vitro assays like DPPH and FRAP,
which showed their inhibitory and reducing potential at 79.17% and 78.42%. Compiling these data, it revealed
that the synthesized AgNPs from Zanthoxylum armatum seed extract have capabilities to be them in biomedical
application