The Potential Therapeutic Advantages of Bacteriophage Targeting MDR Pseudomonas aeruginosa using the Infection Model of Galleria mellonella

The Potential Therapeutic Advantages of Bacteriophage Targeting MDR Pseudomonas aeruginosa using the Infection Model of Galleria mellonella Kandhan Pooncholai https://orcid.org/0009-0001-1670-5705 Suresh Dhanaraj https://orcid.org/0000-0001-8762-1704 Aim:

The growing problem of antibiotic-resistant bacteria in clinical environ-ments has driven increased interest in bacteriophage therapy, where viruses specifically target and destroy harmful bacteria. This research explores the therapeutic potential of bac-teriophages isolated to combat Multi-drug-resistant Pseudomonas aeruginosa, using Gal-leria mellonella larvae as an experimental model. In vitro susceptibility to multiple antibi-otics was assessed using the double agar overlay technique. Additionally, the survival rate of the larvae was analyzed to determine the phages' capacity to combat bacterial infection in G. mellonella.
Introduction:

Resistance to commonly used antibiotics has been steadily increasing over the past few decades, and it has been observed to vary depending on the alternative method employed, such as Bacteriophage Therapy.
Materials and Methods:

The study focused on the Pseudomonas aeruginosa strain PP31, a Multidrug-Resistant Gram Negative bacterium that was obtained from biomedical waste at ICF Hospital in Tamil Nadu, India. Galleria mellonella larvae infected with this multidrug-resistant strain of Pseudomonas were employed for treatment using bacteriophage.
Results:

It was found that a single phage might infect a particular strain of bacteria in the host. It was demonstrated that MDR Pseudomonas aeruginosa infected larvae must be treated with a single specific phage dose (20μL, 104 PFU/mL) at 6 – hour intervals in order to achieve a 95% survival rate for In vivo research. By counting the number of germs in the larvae, the results were confirmed.
Conclusion:

Our research shows that although phages were shown to be highly contagious in vitro, specific phage dosages were required for effective treatment in living animals.
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